Aptamer Details

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Yeast phenylalanine tRNA (B2) (ID# 7459)

Aptamer Chemistry: RNA

Target: Yeast phenylalanine tRNA

Antigen/Target Category: Small Organic

Affinity (Kd): 12 nM (reported value)

Binding Conditions/Buffer: Selection Buffer (250 mM NaCl, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM spermidine, 0.2 mM EDTA)

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 109

Molecular Weight: 35011.9

Extinction Coefficient: 1060400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Scarabino, Daniela, et al. "tRNA prefers to kiss." EMBO Journal 18 (1999): 4571-4578

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