Aptamer Details

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Interferon-? (2’NH2-30) (ID# 7469)

Aptamer Chemistry: DNA

Target: Interferon-y/IFN-y/IFNG

Antigen/Target Category: Protein

Affinity (Kd): 2.7 nM (reported value)

Binding Conditions/Buffer: Binding Mixture: PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na₂HPO₄, and 1.1 mM KH₂PO₄, pH 7.4) containing 1 mM Mg²⁺ ions and 0.01% human serum albumin.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This is a biphasic binding aptamer described by a two-Kd model, 2.7/103 nM.

This aptamer inhibited the binding of IFN-? to its receptor with a ID50 value of ~10 nm. It also inhibited IFN-?-mediated induction of MHC class 1 Ag and CD54(ICAM-1) expression in human myeloid leukemia cells.

The highlighted region in the structure represents the minimal effective sequence. However, the sequence could not be transcribed and no binding information was available.

Sequence ():

Length: 70

Molecular Weight: 38579.5

Extinction Coefficient: 710100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kubik, Mark F., et al. "Isolation and Characterization of 2ƒ??-Fluoro-, 2ƒ??-Amino-, and 2ƒ??-Fluoro-/Amino-Modified RNA Ligands to Human IFN-7 That Inhibit Receptor Binding." Journal of Immunology, 159 (1997): 259-267.

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