Aptamer Details

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HIV-1 TAR RNA Hairpin Loop (B22-19) (ID# 7473)

Aptamer Chemistry: DNA

Target: HIV-1 TAR RNA Hairpin Loop

Antigen/Target Category: Small Organic

Affinity (Kd): 50 nM (reported value)

Binding Conditions/Buffer: R buffer (20 mM HEPES (pH 7.4), 140 mM potassium acetate, 20 mM sodium acetate, 3 mM magnesium acetate)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 19

Molecular Weight: 5659.71

Extinction Coefficient: 166500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sekkai et al. "In Vitro Selection of DNA Aptamers Against the HIV-1 TAR RNA Hairpin." Antisense and Nucleic Acid Drug Development, 12 (2002): 265-274.

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