Aptamer Details

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CCRF-CEM (sgc8) (ID# 7479)

Aptamer Chemistry: DNA

Target: Acute lymphoblastic leukemia (CCRF-CEM)

Antigen/Target Category: Cells

Affinity (Kd): 0.8 nM (reported value)

Binding Conditions/Buffer: Binding buffer: 0.1mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl₂ in Dulbecco's PBS with CaCl₂ and MgCl₂)

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Recognizes and binds to cell lines Molt-4 (T cell Acute Lymphoblastic Leukemia [ALL]), Sup-T1 (T cell ALL), and Jurkat (T cell ALL).

Comments: This aptamer was found to bind to protein tyrosine kinase 7 (PTK7) (Shangguan 2008).

Sequence ():

Length: 88

Molecular Weight: 27106.7

Extinction Coefficient: 859200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shangguan et al. ƒ??Aptamers evolved from live cells as effective molecular probes for cancer study.ƒ? Proceedings of the National Academy of Sciences of the United States of America, 103 (2006): 11838-43.
Sequence was given in:
Shangguan et al. "Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines." Chem. Biochem., 8, (2007): 603-6.

Target protein identification:
D Shangguan et al. Cell-specific aptamer probes for membrane protein elucidation in cancer cells. J. Proteome Res. 7:2133-2139.

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