Aptamer Details

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Acute myeloid leukemia cells (KH1C12) (ID# 7483)

Aptamer Chemistry: DNA

Target: Acute myeloid leukemia cells (HL60)

Antigen/Target Category: Cells

Affinity (Kd): 4.5 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (4.5 g/L glucose, 5 mM MgCl₂, 0.1 mg/ml tRNA and 1mg/ml BSA, dissolved in Dulbecco's PBS with MgCl₂ and CaCl₂) containing 20% FBS.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 68

Molecular Weight: 20896.62

Extinction Coefficient: 647700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sefah, Kwame, et al. "Molecular recognition of acute myeloid leukemia using aptamers." Leukemia, 23 (2009):235-244

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