Aptamer Details

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PrP Fibrils (SAF-93) (ID# 7487)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: PrP Fibrils

Antigen/Target Category: Protein

Affinity (Kd): 23.4 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (20 mM Hepes (pH 7.2), 100 mM NaCl, 50 mM KCl, 10 mM MgCl₂)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: The oligonucleotides used in this study were documented in this reference: Tahiri-Alaoui, A., et al. "High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands." Nucleic Acids Research, 30 (2002): e45

Sequence ():

Length: 116

Molecular Weight: 37278.21

Extinction Coefficient: 1146800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Rhie, A., et al. "Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion." Journal of Biological Chemistry, 278 (2003): 39697-705. https://doi.org/10.1074/jbc.M305297200 Mollasalehi N., Francois-Moutal L., Porciani D., Burke D.H., & Khanna M. (2022). Aptamers Targeting Hallmark Proteins of Neurodegeneration. Nucleic Acid Therapeutics, 32(4). https://doi.org/10.1089/nat.2021.0091

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