Aptamer Details

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PSMA Aptamer (A10-3) (ID# 7498)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: PSMA

Antigen/Target Category: Protein

Ki: 20.5 nM (reported value)

Binding Conditions/Buffer: HEPES buffered saline (pH 7.4), 1 mM MgCl₂, 1 mM CaCl₂, 0.01% I-block (Tropix Inc. Bedford, MA), and 0.05% Tween 20. Incubated at 37 °C for 30 minutes. Washed using the above buffer at 37 °C.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Truncated aptamer is given. A higher K_i was found for the full-length aptamer; see reference for details.

Sequence ():

Length: 56

Molecular Weight: 17966.62

Extinction Coefficient: 539200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lupold, Shawn, et al. "Identification and Characterization of Nuclease-stabilized RNA Molecules That Bind Human Prostate Cancer Cells via the Prostate-specific Membrane Antigen." American Association for Cancer Research Journal 62, (2002): 4029-4033

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