Aptamer Details

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Cyanocobalamin (35-mer) (ID# 7513)

Aptamer Chemistry: RNA

Target: Cyanocobalamin

Antigen/Target Category: Small Organic

Affinity (Kd): 88 nM (reported value)

Binding Conditions/Buffer: Column Buffers (1 M LiC1, 5 mM MgC12, and 25 mM HEPES (pH 7.4) or 1 M NaCl, 5 mM MgC12, and 25 mM HEPES (pH 7.4))

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 35

Molecular Weight: 11263.9

Extinction Coefficient: 341600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lorsch and Szostak. "In vitro selection of RNA aptamers specific for cyanocobalamin." Biochemistry, 33 (1994): 973-982.

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