Aptamer Details

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L-Isoleucine (IL 42-32b) (ID# 7518)

Aptamer Chemistry: RNA

Target: L-Isoleucine

Antigen/Target Category: Small Organic

Affinity (Kd): 200 µM (reported value)

Binding Conditions/Buffer: Selection Buffer (10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl) Reaction Buffer (50 mM sodium borate (pH 8.0), 42 mM KCl, 7.5 mM MgCl2, and 0.4 mM ZnCl2)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 32

Molecular Weight: 10233.16

Extinction Coefficient: 310800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Majerfeld and Yarus. "Isoleucine:RNA sites with associated coding sequence." RNA, 4 (1998): 471-478.

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