Aptamer Details

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Hepatitis C NS3 Protein (10G-1) (ID# 7535)

Aptamer Chemistry: RNA

Target: Hepatitis C NS3 Protein

Antigen/Target Category: Protein

Affinity (Kd): 650 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 50 mM Tris/HCl (pH 7.7), 30 mM NaCl, 5 mM CaCl₂, 10 mM dithiothreitol and 1% BSA Elution Buffer (200 uL of 0.4 M sodium acetate, 5 mM EDTA, and 7 M urea (pH 5.5))

Binding Temp: 23°C

Refolding Protocol: The RNA pool was denatured at 90°C for 2 min and allowed to cool at room temperature for 10 min

Comments: An assay of the inhibition of helicase activity by the aptamer showed an IC50 of 12.5 nM.

Sequence ():

Length: 66

Molecular Weight: 21495.01

Extinction Coefficient: 665000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Urvil, P., et al. "Selection of RNA aptamers that bind specifically to the NS3 protease of hepatitis C virus." European Journal of Biochemistry, 248(1997):130-138.

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