Aptamer Details

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Keratinocyte Growth Factor (14F) (ID# 7542)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Keratinocyte Growth Factor (KGF)

Antigen/Target Category: Protein

Affinity (Kd): 0.3 pM (reported value)

Binding Conditions/Buffer: Binding Buffer (DPBS, 0.5 mM MgCl2, 0.2% BSA, 0.02% sodium azide for PC-3, serum-free MEM growth medium, 0.1% BSA, 25 mM HEPES (pH 7.4), 1 ug/ml heparin for NIH3T3/KGFR)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 69

Molecular Weight: 22180.13

Extinction Coefficient: 664400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Pagratis, N., et al. "Potent 2'-amino-, and 2'-fluoro-2'- deoxyribonucleotide RNA inhibitors of keratinocyte growth factor." Nature Biotechnology, 25(1997):68-73.

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