Aptamer Details

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Pepocin (9-41U22) (ID# 7572)

Aptamer Chemistry: RNA

Target: Pepocin

Antigen/Target Category: Protein

Affinity (Kd): 18 nM (reported value)

Binding Conditions/Buffer: Binding buffer: 3 mM NaH₂PO₄, 3 mM MgCl₂, 45 mM NaCl, 2.5 mM EDTA, 3 mM Tris-HCl (pH 7.5), 0.15 mM DTT, and 12% glycerol.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 41

Molecular Weight: 13103.89

Extinction Coefficient: 410700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Hirao et al. "RNA Aptamers That Bind to and Inhibit the Ribosome-inactivating Protein, Pepocin." The Journal of Biological Chemistry, 275(2000): 4943-4948.

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