Aptagen, LLC
Human Neutrophil Elastase (DNA I) (ID# 7576)
DNA
Human Neutrophil Elastase (HNE)
Protein
17 nM (reported value)
Binding buffer: 150 mM NaCl, 100 mM Tris HCl (pH 7.0), 2 mM MgCl2, 6 mM KCl
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This truncated sequence forms a G-quartet. Neither the full-length, nor the truncated sequence inhibited HNE enzymatic activity, as the aptamer binding site differs from the active site and the substrate binding site.
5'-dTpdApdGpdCpdGpdApdTpdApdCpdTpdGpdCpdGpdTpdGpdGpdGpdTpdTpdGpdGpdGpdGpdCpdGpdGpdGpdTpdApdGpdGpdGpdCpdCpdApdGpdCpdApdGpdTpdCpdTpdCpdGpdTp-3'
45
14046.12
428800
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lin et al. "Peptide conjugation to an in vitro-selected DNA ligand improves enzyme inhibition." PNAS, 92(1995): 11044-11048.
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