Aptamer Details

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Phospholamban (Apt-9) (ID# 7584)

Aptamer Chemistry: DNA

Target: Phospholamban

Antigen/Target Category: Protein

Affinity (Kd): 20 nM (reported value)

Binding Conditions/Buffer: 10 mL of PBS (0..5% Tween 20) containing bead-bound PDZ-Myc-His₆ (5 uL) and 1 ug/mL BSA was incubated for 30 minutes with library.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 40

Molecular Weight: 12546.21

Extinction Coefficient: 400500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tanaka et al. "In Vitro Selection and Characterization of DNA Aptamers Specific for Phospholamban." The Journal of Pharmacology and Experimental Therapeutics, 329(2009): 57-63.

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