Aptamer Details

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Endothelial Cells (P36) (ID# 7594)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: Endothelial Cells

Antigen/Target Category: Cells

Affinity (Kd): 2.2 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (1 mM glucose, 1% BSA, 10 mM MgSO4) Selection Buffer (0.1% BSA, 1 mM CaCl2, and 10 mM MgCl2)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 12

Molecular Weight: 1667.95

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Herrmann et al. "Selection of cell specific peptides in a rat carotid injury model using a random peptide-presenting bacterial library." Bichemica et Biophysica Acta, 1472(1999): 529-536.

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