Aptamer Details

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ErbB2 (SE15-8) (ID# 7597)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: ErbB2 in Breast Cancer Cells

Antigen/Target Category: Protein

Affinity (Kd): 3.49 nM (reported value)

Binding Conditions/Buffer: Binding buffer: 20 mM HEPES (pH 7.0), 150 mM NaCl, 1 mM MgCl₂, 2 mM DTT, and 40 U RNase Inhibitor

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: MDA-MB-453 breast cancer cell line, T47D breast cancer cells, and KPL-4 breast cancer cells (allErbB2 positive)showed staining, while ErbB2 negative cell line MCF-7 did not.

Sequence ():

Length: 34

Molecular Weight: 11239.85

Extinction Coefficient: 341100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kim and Jeong. "In Vitro Selection of RNA Aptamer and Specific Targeting of ErbB2 in Breast Cancer Cells." Oligonucleotides, doi:10.1089/oli.2011.028.

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