Aptamer Details

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PC12 Cells (Aptamer17a) (ID# 7618)

Aptamer Chemistry: DNA

Target: PC12 Cells

Antigen/Target Category: Cells

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Washed with selection buffer (5 mM MgCl in PBS) from and 1 mg/ml yeast tRNAfor 30 minutes.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Binding conditions from Wang et al. (2003) and truncated aptamer 17 (Aptamer 17a) from Liu et al. (2011).

Sequence ():

Length: 25

Molecular Weight: 7954.23

Extinction Coefficient: 255700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Liu et al. "Dissection of the Functional Structure of Aptamer17, Which Specifically Recognizes Differential PC12 Cells." Nucleic Acid Therapeutics, 21(2011): 225-229.
Wang et al. "Single-stranded DNA Aptamers thatBbind Differentiated but not Parental Cells: Subtractive Systematic Evolution of Ligands by Exponential Enrichment." J. Biotechnol., 102(2003): 15-22.

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