Aptamer Details

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Escherichia coli (E. coli) O157:H7 (ID# 7660)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Chimeric

Target: Escherichia coli (E. coli) O157:H7

Antigen/Target Category: Cells

Affinity (Kd): 110 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (30 mM Trisƒ??HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, and 1% BSA). Incubated at 37°C for 24 hours.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 64

Molecular Weight: 20656.14

Extinction Coefficient: 662700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Lee at al. "In vitro selection of Escherichia coli O157:H7-specific RNA aptamer." Biochemical and Biophysical Research Communications, 417(2012): 214-220.

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