Aptamer Details

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M64RA (ID# 7669)

Aptamer Chemistry: DNA

Target: MPT64 Protein

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: SHCMK binding buffer: 20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl₂, and 1 mM MgCl₂

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Was used in a sandwich assay with M64CA.

Sequence ():

Length: 35

Molecular Weight: 10873.13

Extinction Coefficient: 339000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Qin, L., et al.(2009). "The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis." Clin Chem Lab Med, 47(4), 405-411. doi: 10.1515/CCLM.2009.097

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