Aptamer Details

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Nucleolin aptamer (AS1411) (ID# 7675)

Aptamer Chemistry: DNA

Target: Nucleolin

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: For fluoresecent labeling, 400 nM aptamer were added to cell growth media for 1 hour at 37°C and 5% CO₂. Dulbecco's phosphate-buffered saline was used to wash cells.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: The image does not reflect the G quartet structure.

Kotula's et al. research suggests that the aptamer is internalized through a nonstandard mechanism.

Sequence ():

Length: 26

Molecular Weight: 8272.41

Extinction Coefficient: 250800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kotula J, Sullenger B, et al. 2012. Aptamer-mediated delivery of splice-switching oligonucleotides to the nuclei of cancer cells. Nucleic Acid and Therapeutics 22.3:187-195. Li, L. et al. 2014. Aptamer photoregulation in vivo. PNAS 11.48: 17099-103.

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