Aptamer Details

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PCB77 (II) (ID# 7689)

Aptamer Chemistry: DNA

Target: 3,3,4,4-tetrachlorobiphenyl (PCB77)

Antigen/Target Category: Small Organic

Affinity (Kd): 8.32 µM (reported value)

Binding Conditions/Buffer: Binding Buffer: 100 mM NaCl, 20 mM Trisƒ??HCl [pH 7.6], 2 mM MgCl₂, 5 mM KCl, 1 mM CaCl₂, and 0.02% Tween 20

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 38

Molecular Weight: 11783.7

Extinction Coefficient: 374200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Shengmin, X. (2012). Selection of dna aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis. Analytical Biochemistry, 423(2012), 195-201.

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