Aptamer Details

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Plasmodium vivax lactate dehydrogenase (pL1) (ID# 7693)

Aptamer Chemistry: DNA

Target: Plasmodium vivax lactate dehydrogenase (PvLDH)

Antigen/Target Category: Protein

Affinity (Kd): 16.8 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 20mM Trisƒ??HCl pH 8.0, 50mM NaCl, 5mM KCl, and 5mM MgCl₂)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: For details on pL1's use in colorimetric testing, see: Lee S, Manjunatha DH, Jeon W, Ban C (2014) Cationic Surfactant-Based Colorimetric Detection of Plasmodium Lactate Dehydrogenase, a Biomarker for Malaria, Using the Specific DNA Aptamer. PLoS ONE 9(7): e100847. doi:10.1371/journal.pone.0100847

Sequence ():

Length: 36

Molecular Weight: 11163.29

Extinction Coefficient: 340000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Seonghwan, L. (2012). A highly sensitive aptasensor towards plasmodium lactate dehydrogenase for the diagnosis of malaria. Biosensors and Bioelectronics, 35(2012), 291-296.

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