Aptamer Details

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Adipose Tissue (adipo-8) (ID# 7708)

Aptamer Chemistry: DNA

Target: Mature 3T3-L1 adipocytes (adipose tissue)

Antigen/Target Category: Cells

Affinity (Kd): 17.8 nM (reported value)

Binding Conditions/Buffer: 4.5 mg/ml glucose and 5 mM MgCl₂ in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Aptamer binds to adipose cells at 4 °C and 37°C. Trypsin assay suggests that aptamer binds to a surface protein.

Sequence ():

Length: 87

Molecular Weight: 27226.7

Extinction Coefficient: 862800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

J. Liu et. al. "Selection of aptamers specific for adipose tissue." PLoS One, 7(2012): e37789.

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