Aptamer Details

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HIV-1 R5 SU Glycoprotein gp120 (B40t77) (ID# 7732)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: HIV-1 R5 gp120

Antigen/Target Category: Protein

Affinity (Kd): 31 nM (reported value)

Binding Conditions/Buffer: cHBS buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM CaCl ₂, 1 mM MgCl₂, 2.7 mM KCl

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: IC= ~2nM in HIV-1_Ba-L. This aptamer is a truncated version of B40.

Sequence ():

Length: 77

Molecular Weight: 24701.55

Extinction Coefficient: 737700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

A. K. Dey et. al. Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5 strains of HIV-1. RNA 11:873-884

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