Aptamer Details

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Tumor-derived endothelial cells (PepMW3) (ID# 7748)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: Tumor-derived endothelial cells (TDECs)

Antigen/Target Category: Cells

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: IMC Medium: 1X M9 Salts (40 mM Na₂HPO₄, 20 mM KH₂PO₄, 8.5 mM NaCl, 20 mM NH₄Cl, pH 7.4), 0.2% casamino acids, 0.5% glucose, 1 mM MgCl₂.
Incubation/Wash solution: IMC medium containing 10% fetal calf serum (FCS) and 1% a-methyl mannoside.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds to TDECs.
Counter-SELEXed against Matrigel invading cells (MAGIC) and minimal binding to NIH3T3 cells.

Sequence ():

Length: 9

Molecular Weight: 960.21

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

C K Brown et al. A novel approach for the identificaiton of unique tumor vasculature binding peptides using an E. coli peptide display library. Ann Surg Oncol. 7(2000): 743-749.

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