Aptamer Details

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Bacillus thuringiensis spores (ID# 7756)

Aptamer Chemistry: DNA

Target: Bacillus thuringiensis spores

Antigen/Target Category: Cells

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: PBS

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Aptamer was conjugated to quantum dots using a 5'-HS-(CH₂)₆ linker.

Sequence ():

Length: 60

Molecular Weight: 18379.9

Extinction Coefficient: 548400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

M Ikanovic et al. Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores. J Fluoresc 17(2007): 193-199

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