Aptamer Details

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Ochratoxin A (H12) (ID# 7757)

Aptamer Chemistry: DNA

Target: Ochratoxin A (OTA)

Antigen/Target Category: Small Organic

Affinity (Kd): 96 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: PBS 1X, 1 mM MgCl₂, 0.01% Tween 20, pH 7.4.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: This aptamer showed minimal cross-reactivity with ochratoxin B and phenylalanine.

Comments: The limit of detection for the enzyme-linked assay was 1 nM with a linear working range from 1-80 nM.

Sequence ():

Length: 66

Molecular Weight: 20569.34

Extinction Coefficient: 649700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

L Barthelmebs et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine. Food Control 22(2011): 737-743

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