Aptagen, LLC
theophylline-inhibited molecular switch (ID# 7763)
RNA
theophylline
Small Organic
na nM (reported value)
Reaction buffer [50 mM Tris.HCl (pH 7.5) and 20 mM MgCl2] for 20 hr
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprCprGprAprCprCprCprUprGprAprUprGprAprGprAprUprGp rAprUprAprCprCprAprGprCprCprGprAprAprAprGprGprCprCprCprUprUprGprGprCprAprGp rCprUprCprUprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprUprGprCprCprCp-3'
75
24231.68
727900
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Garrett A. Soukup and Ronald R. Breaker. "Engineering precision RNA molecular switches" Proc. Natl. Acad. Sci. 96(1999):3584-3589.
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