Aptamer Details

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Neomycin (LGA11) (ID# 7795)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Neomycin and other aminogycosides (AMGs)

Antigen/Target Category: Small Organic

Affinity (Kd): 40.3 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: PBS with 10% (v/v) methanol

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds to several aminoglycosides: kanamycin, paromycin streptomycin, apramycin, tobramycin, neomycin, and gentamycin.

Comments: See also aptamer LS13 which targets the same aminoglycosides.

Sequence ():

Length: 79

Molecular Weight: 25568.23

Extinction Coefficient: 771400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

N. Derbyshire et al. Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays. Anal. Chem. (2012) 84: 6595-6602.

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