Aptamer Details

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AMPA GluR2 (AN58) (ID# 7809)

Aptamer Chemistry: RNA

Target: AMPA GluR2 Glutamate Receptor Channel

Antigen/Target Category: Protein

Affinity (Kd): 419 pM (reported value)

Binding Conditions/Buffer: 10% DMSO in Extracellular Buffer: 150 mM NaCl, 3 mM KCl, 1 mM CaCl₂, 1 MgCl₂, 10 HEPEs (pH 7.4)
Incubation Time: 40 min

Binding Temp: 22°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This aptamer forms two different structures, both of which bind to GluR2 and both of which are necessary to inhibit the receptor channel. See Huang (2009) for more details.

Sequence ():

Length: 58

Molecular Weight: 18676.31

Extinction Coefficient: 584600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Z. Huang et al. RNA aptamers selected against the GluR2 glutamate receptor channel. Biochemistry 46(2007): 12648 - 12655.
Z. Huang et al. One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors. Nucleic Acids Res.. 37(2009): 4022 - 4032

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Aptamer Details

AMPA GluR2 (AN58) (ID# 7809)

Z. Huang et al. RNA aptamers selected against the GluR2 glutamate receptor channel. Biochemistry 46(2007): 12648 - 12655. Z. Huang et al. One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors. Nucleic Acids Res.. 37(2009): 4022 - 4032

Z. Huang et al. RNA aptamers selected against the GluR2 glutamate receptor channel. Biochemistry 46(2007): 12648 - 12655.
Z. Huang et al. One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors. Nucleic Acids Res.. 37(2009): 4022 - 4032