Aptamer Details

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C-Reactive Protein (CRP1-1) (ID# 7818)

Aptamer Chemistry: RNA

Target: C-Reactive Protein (CRP)

Antigen/Target Category: Protein

Affinity (Kd): 2.25 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 20 mM Tris-HCl (pH 7.5), 140 mM NaCl, 2 mM CaCl₂ and 1 mM MgCl₂.
Incubation Time: 30 min.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binding experiments indicate that CaCl₂ is necessary for binding, suggesting that the aptamer targets the pentamer form of CRP or that the aptamer itself requires CaCl₂ to bind.

Sequence ():

Length: 104

Molecular Weight: 33559.36

Extinction Coefficient: 1033300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

N Orito, Y Kikuchi, et al. High-affinity RNA aptamers to C-reactive protein (CRP): newly developed pre-elution methods for aptamer selection. J. Phys. Conf. Ser. 352(2012): 012042. doi:10.1088/1742-6596/352/1/012042.

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