Aptamer Details

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p-Nosyl Protected Alkyl Amino Group (M6b-M14) (ID# 7824)

Aptamer Chemistry: DNA

Target: p-nosyl protected alkyl amino groups

Antigen/Target Category: Small Organic

Affinity (Kd): 2.27 nM (reported value)

Binding Conditions/Buffer: Optimized Buffer: 10 mM Na₂HPO₄, 2 mM KH₂, 500 mM NaCl, pH 7.4.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Competition binding assay performed by Mei et al. showed that the aptamer requires the entire p-nosyl-amide moiety.

Comments: Forms an anti-parallel G-quadruplex.

Sequence ():

Length: 32

Molecular Weight: 9917.5

Extinction Coefficient: 306300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Mei H et al. (2012) Functional-group specific aptamers indirectly recognizing compounds with alkyl amino group. Anal. Chem. 84: 7323 - 7329

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