Aptagen, LLC
Arsenic(III) (Ars-3) (ID# 7825)
DNA
Arsenic(III)
Small Organic
4.95 nM (reported value)
Selection buffer: 150 mM NaCl, 50mM HEPES, 0.5 mM MgCl2, 0.5 mM CaCl2
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Wu et al. converted this aptamer into a biosensor that could colorimetrically detect As(III) at concentrations as low as 10 ppb when using Hexadecyltrimethylammonium bromide (CTAB) as the cationic surfactant, or as low 5 ppb when using hexadecylpyridinium chloride monohydrate (HPCM) as the cationic surfactant.
5'-dGpdGpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdGpdApdGpdApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdTpdTpdApdCpdApdGpdApdApdCpdApdApdCpdCpdApdApdCpdGpdTpdCpdGpdCpdTpdCpdCpdGpdGpdGpdTpdApdCpdTpdTpdCpdTpdTpdCpdApdTpdCpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
100
30767.08
981200
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kim, et al. "Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer." Environ. Sci. Technol., 43 (2009): 9335-9340. Wu et al. "Ultrasensitive Aptamer Biosensor for Arsenic(III) Detection in Aqueous Solution Based on Surfactant-Induced Aggregation of Gold Particles." Analyst, 137 (2012): 4171-4178.
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