Aptamer Details

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Pro-Gastrin Releasing Peptide (ID# 7836)

Aptamer Chemistry: DNA

Target: Pro-gastrin releasing peptide (proGRP)

Antigen/Target Category: Protein

Affinity (Kd): 1.2 µM (reported value)

Binding Conditions/Buffer: SELEX buffer used in SPR binding assay: 137 mM NaCl, 8.1 mM Na₂HPO₄, 2.68 mM KCl, 1.47 mM KH₂PO₄, 0.9 mM CaCl₂, 5.3 mM MgCl₂, and 4.5 g/L glucose (pH 7.4)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 61

Molecular Weight: 18685.27

Extinction Coefficient: 593700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

M Mie et al. (2012) Selection of DNA aptamers with affinity for Pro-gastrin-Releasing Peptide (proGRP), a tumor marker for small cell lung cancer. Appl. Biochem. Biotechnol. Published Online ahead of Print: DOI 10.1007/s12010-012-9956-5

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