Aptamer Details

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Ampicillin (AMP17) (ID# 7854)

Aptamer Chemistry: DNA

Target: Ampicillin

Antigen/Target Category: Small Organic

Affinity (Kd): 13.4 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl₂.

Binding Temp: RT°C

Refolding Protocol: Heated at 90°C for 3 min and then cooled at 4°C for 1 hour.

Specificity: Did not bind to related antibiotics amoxicillin and benzylpenicillin, or unrelated antibiotics chloramphenicol, tetracycline, sulfadimethoxine, streptomycin, and kanamycin.

Comments: Because of AMP17's ability to aggregate on gold nanoparticles at lower salt concentrations than the other two aptamers, AMP17 was further evaluated in a colorimetric-fluorometric assay.

Sequence ():

Length: 19

Molecular Weight: 5940.9

Extinction Coefficient: 183800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 402:2153-2161.

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