Aptamer Details

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Mycoplasma bovis detection aptamer (WKB-14) (ID# 7899)

Aptamer Chemistry: DNA

Target: P48 of M. Bovis

Antigen/Target Category: Protein

Affinity (Kd): 15.61 nM (reported value)

Binding Conditions/Buffer: Added to wells of plate coated with P48 protein solution (P48 protein dissolved in 100 uL of 0.05 carbonate-bicarbonate buffer)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Relatively similar to mAB 10E to P48

Sequence ():

Length: 80

Molecular Weight: 24687.08

Extinction Coefficient: 769800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Fu, P., et. al. Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serum.

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