Aptamer Details

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Clone 2 (ID# 7902)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Protein E (PE)

Antigen/Target Category: Protein

Affinity (Kd): 1.2 nM (reported value)

Binding Conditions/Buffer: 165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Niƒ??NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2)

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 66

Molecular Weight: 20369.18

Extinction Coefficient: 643700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12.

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