Aptagen, LLC
Clone 488 (ID# 7915)
RNA
SelB
Protein
50 mM potassium phosphate, pH 7.0, 5 mM Mg(OAc)2, 0.1 mM EDTA, 1 mM DTT, 0.5 mM GTP,0.02% Tween 20, 400 U/mL RNAsin
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
dGpdCpdGpdCpdTpdApdApdGpdTpdCpdCpdTpdCpdGpdCpdTpdCpdApdGpdCpdCpdCpdApUdApdApdGpUUdGpUdCpdCpdCpdApdApdGpUdCpUUdGpdGpdGpdCpdGpdCpdApdApdApUdApdCpdApUdCpdCpdCpdApdCpdGpdCpdGpdCpdGpdApdCpdTpdCpdGpdGpdApdTpdCpdCpdGp
67
20508.24
621000
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Klug, S. J. et al. "In vitro selection of RNA aptamers that bind special elongation factor SelB, a protein with multiple RNA-binding sites, reveals one major interaction domain at the carboxyl terminus." RNA, 1995 5: 1180 - 1190.
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