Aptamer Details

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Atrazine R12.23 (ID# 7936)

Aptamer Chemistry: DNA

Target: Atrazine

Antigen/Target Category: Small Organic

Affinity (Kd): 0.62 nM (reported value)

Binding Conditions/Buffer: 500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride

Binding Temp: 24°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Analysis showed significantly higher binding of R12.23 to atrazine in solution than to four of the five negative targets used in the selection (see Table 3 of source material)

Sequence ():

Length: 79

Molecular Weight: 24464.91

Extinction Coefficient: 749900

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332

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