Aptamer Details

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EpCAM EP166 (ID# 7939)

Aptamer Chemistry: DNA

Target: EpCAM

Antigen/Target Category: Protein

Affinity (Kd): 5.48 nM (reported value)

Binding Conditions/Buffer: Binding buffer [4.5 g/L glucose, 5 mM MgCl2 and 10% FBS in Dulbeccoƒ??s PBS (Sigma)] Bovine Serum Albumin (1 mg/ml; Thermo Inc., USA)

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds strongly HepG2 cells expressing EpCAM but only binds very weakly to HepG2 cells that do not express EpCAM

Sequence ():

Length: 231

Molecular Weight: 23973.58

Extinction Coefficient: 767300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kim, J., W.; Kim, E., Y.; Kim, S., Y; Byun, S., K.; Lee, D.; Oh, K.; Kim, W., K.; Han, B., S.; Chi, S.; Lee, S., C.; Bae, K. Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX. Molecules and Cells, 2014, 37(10), 742-746 ISSN: 0219-1032

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