Aptamer Details

Back to Search Results

Uranium DNAzyme (ID# 7950)

Aptamer Chemistry: DNA

Target: Uranium

Antigen/Target Category: Other

Affinity (Kd): n/a nM (reported value)

Binding Conditions/Buffer: 0.4 M NaCl and 20 mM MES buffer solution (pH 5.5) at 20?C for 15 min. To quench: 5 uL of 0.5 M Tris base solution added then sample was vortexed to shift the pH from 5.5 to 8.0.

Binding Temp: 20°C

Refolding Protocol: 1.0 mL 9.6 ?? 10-5 M DNAzyme 1.0 mL 6.4 ?? 10-5 M TAMRA-modified substrate Mixed with 1.0 mL of 2.0 M NaCl and 1.0 mL of 20 mM MES buffer solution (pH 5.5). Heat at 90?C for 15 min and cooled down t

Specificity: Tested against Barium, Calcium, Cadmium, Chromium, Iron 2+, Iron 3+, Mercury, Magnesium, Manganese, Nickel, and Lead. Even in 100-fold excess concentrations over that used for Uranium, fluorescence intensity did not increase significantly, except in the presence of Uranium.

Comments: Limit of Detection: 0.41 nM Uranium Substrate Sequence: TAMRA-CCTC ACTAT rA GGAAG AGATG GACGT G

Sequence ():

Length: 54

Molecular Weight: 16630.84

Extinction Coefficient: 524500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Sai Jin Xiao, Jun Zuo, Zhi Qiang Zhu, Yong Zhong Ouyang, Xing Lei Zhang, Huan Wen Chen, & Li Zhang (2015) Highly Sensitive DNAzyme Sensor for Selective Detection of Trace Uranium in Ore and Natural Water Samples. Sensors and Actuators B 210 (2015) 656-660

Have your aptamer oligo synthesized ORDER