Aptamer Details

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Botulinum neurotoxin type A (BoNT A) Aptamer (ID# 7952)

Aptamer Chemistry: DNA

Target: BoNT A Light Chain (BoNT/A-rLc)

Antigen/Target Category: Protein

Affinity (Kd): 34 nM (reported value)

Binding Conditions/Buffer: 1X Binding Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM KCl, 2 mM MgCl2, 2mM CaCl2 (pH 7.4)

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 60

Molecular Weight: 18544.08

Extinction Coefficient: 547900

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

(1) Bogomolova, A., Aldissi, M. ƒ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.ƒ? Biosensors and Bioelecgtronics, 66 (2015): 290-296. (2) Lou, X., et al. ƒ??Micromagnetic selection of aptamers in microfluidic channels.ƒ? PNAS, 106, 9 (2009): 2989-2994.

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