Aptamer Details

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Beta-Estradiol Aptamer (BES.1) (ID# 7961)

Aptamer Chemistry: DNA

Target: Beta-Estradiol (Estrogen)

Antigen/Target Category: Small Organic

Affinity (Kd): 6 µM (reported value)

Binding Conditions/Buffer: 1X Binding Buffer: 20 mm Tris (pH 7.4), 1 M NaCl, 10 mM MgCl₂

Binding Temp: 23°C

Refolding Protocol: 95°C for 5 minutes, allow aptamer to cool to room temperature over 10 minutes

Specificity: Specific to beta-estradiol over deoxycholic acid. Shows cross-reactivity towards dehydroisoandrosterone 3-sulfate and deoxycorticosterone 21-glucoside.

Comments: Affinity estimated based on fluorescence response data provided in Supplementary Table 4.

Sequence ():

Length: 48

Molecular Weight: 14720.56

Extinction Coefficient: 446300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors. J. Am. Chem. Soc.134:1642-1647.

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