Aptamer Details

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Thrombin 03 (ID# 7962)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): 7.03 pM (reported value)

Binding Conditions/Buffer: PBSMCT (DPBS with 2.5 mM MgCl2, 1 mM CaCl2, 0.05% TWEEN-20)

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 60

Molecular Weight: 18794.24

Extinction Coefficient: 585800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, J., Gong, Q., Maheshwari, N., Eisenstein, M., Luz Arcila, M., Kosik, K., & Soh, T. Particle Display: A Quantitative Screening Method for Generating High-Affinity Aptamers. Angew. Chem. Int. Ed. 2014, 53, 4796ƒ??4801

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