Aptagen, LLC
aptamer 1 (ID# 8019)
DNA
SARS-CoV nucleocapsid, SARS-CoV-2 nucleocapsid
Protein
4.93±0.30 nM (reported value)
50 mM Tris/Cl, pH 8.0, 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol (DTT) and 1% (w/v) BSA
Room Temp°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Bind to both SARS-CoV and SARS-CoV-2 Nucleocapcid proteinNot bind to SARS-CoV-2 spike protein receptor binding domain, or SARS-Cov-2 3C-like protease
5'-dGpdCpdApdApdTpdGpdGpdTpdApdCpdGpdGpdTpdApdCpdTpdTpdCpdCpdGpdGpdApdTpdGpdCpdGpdGpdApdApdApdCpdTpdGpdGpdCpdTpdApdApdTpdTpdGpdGpdTpdGpdApdGpdGpdCpdTpdGpdGpdGpdGpdCpdGpdGpdTpdCpdGpdTpdGpdCpdApdGpdCpdApdApdApdApdGpdTpdGpdCpdApdCpdGpdCpdTpdApdCpdTpdTpdTpdGpdCpdTpdApdAp-3'
88
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Cho, S. et al. "Novel system for detecting SARS coronavirus nucleocapsid protein using an ssDNA aptamer". Journal of Bioscience and Bioengineering, 112(2011):535-540.
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