Aptamer Details

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DE3A (ID# 8020)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: Sickle Hemoglobin

Antigen/Target Category: Protein

Affinity (Kd): 1.68 µM (reported value)

Binding Conditions/Buffer: 20 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, 0.01% BSA

Binding Temp: 20°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: DE3A showed nearly identical bindingaffinities to FmetHbS and oxyHbS, with respective Kd valuesof 1.68 and 1.74 mM.

Sequence ():

Length: 80

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Purvis, S. H., Keefer, J. R., Fortenberry, Y. M., Barron-Casella, E. A., & Casella, J. F. (2017). Identification of Aptamers That Bind to Sickle Hemoglobin and Inhibit Its Polymerization. Nucleic Acid Therapeutics, 27(6), 354–364. doi:10.1089/nat.2016.0646

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