Aptamer Details

Back to Search Results

PSMA Dimeric Aptamer (ID# 8027)

Aptamer Chemistry: DNA

Target: Prostate Specific Membrane Antigen (PSMA)

Antigen/Target Category: Protein

Binding Conditions/Buffer: SELEX forward rounds were performed by adding 1 mg of PSMA bound to Dynabeads Talon (Invitrogen, Oslo, Norway) to 700 μl of binding buffer (100 mmol/l NaCl, 20 mmol/l Tris, 2 mmol/l MgCl2, 5 mmol/l KCl, 1 mmol/l CaCl2, 0.2% Tween-20, pH 8). The beads were removed using a Dynal magnet (Invitrogen) and were washed four times with binding buffer. At least 10 μg of ssDNA was annealed by heating to 95 °C followed by gentle cooling and was added to the PSMA matrix followed by vortexing and incubation for 1 hour at 37 °C with mild agitation.

Binding Temp: 25°C

Refolding Protocol: At least 10 μg of ssDNA was annealed by heating to 95 °C followed by gentle cooling and was added to the PSMA matrix followed by vortexing and incubation for 1 hour at 37 °C with mild agitation.

Comments: Dimeric aptamer anneals with partial complement poly-T16 sequence.

Sequence ():

Length: 72

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Boyacioglu, O., Stuart, C. H., Kulik, G., & Gmeiner, W. H. (2013). Dimeric DNA Aptamer Complexes for High-capacity-targeted Drug Delivery Using pH-sensitive Covalent Linkages. Molecular therapy. Nucleic acids, 2(7), e107. https://doi.org/10.1038/mtna.2013.37

Have your aptamer oligo synthesized ORDER