Aptamer Details

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2mHNE-5 (ID# 8031)

Aptamer Chemistry: DNA

Target: HNE

Antigen/Target Category: Protein

Affinity (Kd): 480 nM (reported value)

Binding Conditions/Buffer: Binding BUffer (20mM HEPES, pH 7.5, 150mM NaCL, 6mM KCL, 2mM MgCl2)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: each dNTP modified with 2'-OMe

Sequence ():

Length: 93

Molecular Weight: 20649.39

Extinction Coefficient: 672360

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Liu, Zhixia, et al. "Evolved polymerases facilitate selection of fully 2'-OMe-modified aptamers" Royal Society of Chemistry, 8 (2017): 8179-8182

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