Aptagen, LLC
s5rev (ID# 8035)
DNA
CD44
Protein
238 nM (reported value)
50 mM NaH2PO4/
Na2HPO4 buffer pH 7.5 containing 300 mM NaCl and 0.01%
(v/v) Tween 20
23°C
The ssDNA pool from theprevious cycle was resuspended in selection buffer, denatured for 5 min at 95C, immediately cooled on ice for10 min, and equilibrated for 10 min at RT.
Tested for specificity against CD44 over MDM2, MDMX, and BSA
5'-dCpdApdTpdGpdCpdTpdTpdCpdCpdCpdCpdApdGpdGpdGpdApdGpdApdTpdGpdApdCpdCpdGpdGpdGpdGpdCpdGpdTpdApdCpdApdCpdCpdGpdTpdCpdGpdCpdGpdGpdCpdApdCpdApdTpdGpdTpdCpdTpdGpdApdApdTpdGpdCpdGpdTpdTpdTpdApdGpdTpdCpdTpdCpdTpdGpdTpdGpdGp-3'
60
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Pęcak, Aleksandra, et al. “Anti-CD44 DNA Aptamers Selectively Target Cancer Cells.” Nucleic Acid Therapeutics, vol. 30, no. 5, 2020, pp. 289–298., doi:10.1089/nat.2019.0833.
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