Aptamer Details

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Aptamer 1 – Switch antisense (ID# 8040)

Aptamer Chemistry: DNA

Target: SARS-CoV nucleocapsid, SARS-CoV-2 nucleocapsid

Antigen/Target Category: Protein

Affinity (Kd): 4.93 nM (reported value)

Binding Conditions/Buffer: 10 mM Tris, 0.5 mM NaCl, 1 mM EDTA, pH 7.4

Binding Temp: 23°C

Refolding Protocol: Main aptamer: 80°C for 3 minutes, gentle cooling at 23°C for 5 minutes.Antisense Apta-switch component: 40°C for 10 minutes, then combine with refolded main aptamer and incubate for 2 hours at 23°C. Wash with 1X DPBS, store at 4°C.

Specificity: N/A. This is an antisense complementary sequence to Nucleocapsid aptamer ID #: 7074.

Comments: Apta-switch component (antisense strand) to Nucleocapsid protein aptamer (ID #: 7074). Originally reported with poly(dT) spacer and linker for functionalization.

Sequence ():

Length: 14

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Singh NK, Ray P, Carlin AF, Magallanes C, Morgan SC, Laurent LC, Aronoff-Spencer ES, Hall DA. "Hitting the diagnostic sweet spot: Pont-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf gluocometer". medRxiv preprint. doi: https://doi.org/10.1101/2020.09.24.20200394.

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